Cristiano Ronaldo denies he swore at Portugal coach over substitution
Ronaldo, Portugal's captain, said the comments he made as he left the field in the 65th minute were in fact aimed at a South Korean player.
Portuguese media said the former Manchester United, Real Madrid and Juventus player, who left the English club last month, appeared to challenge Santos over the decision to substitute him, using vulgar language towards the coach.
"What happened was before my substitution, the South Korea player told me to get off quickly. I told him to shut up as he doesn't have that authority. There was no disagreement (with the coach)," Ronaldo was quoted as saying by Portuguese reporters.
He is seeking to equal the Portuguese record of most World Cup goals held by Eusebio, who racked up nine in 1966 in England, the only World Cup tournament he played in.
Ronaldo last week became the first ever player to score in five World Cups.
Santos, asked by reporters about the substitution incident, also said a South Korean player had been aggressive in his comments towards the five-time Ballon d'Or winner.
"I saw the interaction with Korean player and I have no doubts about what happened," Santos said.
Ronaldo was kept largely at bay by South Korea's defence in the Asians' last-gasp victory over Portugal that sent Uruguay out of the World Cup.
But he was involved in the South Korean equalizer in the 27th minute when a corner struck him on the back and fell for Kim Young-gwon to score.
Santos made six changes to the side that beat Uruguay on Monday, mindful of the risk of yellow-card suspensions in the next round for which Portugal had already qualified before kick-off against South Korea.
"We're unhappy. We wanted to go through with a win, we wanted to go through playing good football to keep boosting our confidence," Santos said.
"We're not going to lose confidence, this group has confidence, but it's a warning, a serious warning. Perhaps some positive things could come from this game."
He congratulated Paulo Bento, his predecessor as Portugal's manager who is now in charge of South Korea.
"I've just given him a hug. I'm unhappy, he is happy. I didn't facilitate anything but friends are friends, business aside," he added.
Portugal, by finishing top of Group H, will play Switzerland in the last 16 and South Korea will take on Brazil.