Tour de France overall leader Jonas Vingegaard (25) suffered a blow on Sunday when one of his key team mates, Steven Kruijswijk (35), abandoned the race after a crash during the 15th stage.
Dutchman Kruijswijk held his right shoulder as he sat on the road after the crash during the 202.5-km ride between Rodez and Carcassonne, before throwing in the towel.
Primoz Roglic, the 2020 runner-up, was the first Jumbo-Visma rider to leave the race when he did not take the start of the stage after failing to recover from injuries sustained in a crash last week.
Jumbo-Visma are now down to six men.